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KMID : 0894520120160020129
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Development & Reproduction
2012 Volume.16 No. 2 p.129 ~ p.135
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Identification of CATHEPSIN B as a Novel Binding Protein of the Cell Death Regulating Protein IEX-1
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Ryou Sang-Mi
Lee Kang-Seok
Bae Jee-hyeon
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Abstract
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Previously, we identified that the overexpression of IEX-1 induces apoptosis in ovarian cancer cells. Herein we report a new binding partner of IEX-1, CATHEPSIN B, as a lysosomal enzyme which contributes to the various apoptotic signaling in tumor cells. To investigate how IEX-1 regulates cellular survival and death event, we performed yeast two-hybrid screening of rat ovarian cDNA library using IEX-1 as the bait and found CATHEPSIN B. In the present study, CATHEPSIN B and IEX-1 proteins were overexpressed in 293T cells and their specific association was determined by immunoprecipitation and immunoblot analysis. In addition, the endogenous interaction between CATHEPSIN B and IEX-1 was confirmed in HeLa cells. The current finding of lysosomal CATHEPSIN B as the IEX-1-binding partner implies that IEX-1 may involve in lysosome-mediated apoptotic signaling pathways.
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KEYWORD
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IEX-1, CATHEPSIN B, Apoptosis
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